Tumor progression during healing study on mice

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Experimental Study on Residual Tumor Angiogenesis after
CryoablationChun-Hua Ma&, Rong Jiang&*, Jin-Duo Li, Bin Wang, Li-Wei Sun, Yuan Lv


Abstract
Objective: To explore the mechanism and significance of tumor angiogenesis by observing changes of microvessel
density (MVD) and expression of vascular endothelial growth factor (VEGF) in residual tumor tissues
after cryoablation. Materials and Methods: A total of 18 nude mice xenograft models with transplanted lung
adenocarcinoma cell line A549 were established and randomly divided into 3 groups when the maximum diameter
of tumor reached 1 cm: control, cisplatin (DDP) and cryoablation. The nude mice were sacrificed after 21-d
cryoablation to obtain the tumor tissues. Then immunohistochemistry was applied to determine MVD and the
expression of VEGF in tumor tissues. Results: The tumor volumes of control group, DDP group and cryoablation
group were 1.48±0.14 cm3, 1.03±0.12 cm3 and 0.99±0.06 cm3 respectively and the differences were significant
(P<0.01), whereas MVD values were 21.1±0.86, 24.7±0.72 and 29.2±0.96 (P<0.01) and the positive expression
rates of VEGF were 36.2±1.72%, 39.0±1.79% and 50.8±2.14% (P<0.01), respectively, showing that MVD was
proportional to the positive expression of VEGF (r=0.928, P<0.01). Conclusions: Cryoablation can effectively
inhibit tumor growth, but tumor angiogenesis significantly increases in residual tumors, with high expression
of VEGF playing an important role in the residual tumor angiogenesis.




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